enzymatic digestion meaning in Chinese
酶消化
Examples
- Determination of disulfide bonds in protein by enzymatic digestion and mass spectrometry
酶切质谱法测定蛋白质的二硫键 - As a result , more than 97 % of the testicular cells remained their viability after enzymatic digestion
结果组合酶消化后获得的睾丸细胞悬液中, 97以上的细胞仍然存活。 - So a lagre populations of relatively purified spermatids can be isolated from mouse testis by enzymatic digestion combined with discontinuous percoll gradient centrifugation method
因此采用组合酶消化结合非连续percoll梯度离。 g法,可有效地从小鼠睾丸中分离到较多较纯的存活精子细胞。 - Most commonly used is the mechanical method in which a small piece of testicular tissue is torn apart using needles and the spermatids are released . while enzymatic digestion of human testicular tissue can result in a mixture of cell types including a whole range of spermatogenic cell types
最常用的是机械方法,即利用细针将活检获得的小块睾丸组织撕开,释放出精子细胞;也可利用酶将睾丸组织消化成细胞悬液,其中包含所有类型的生精细胞。 - In the first trial , combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis , and then a modified discontinuous percoll gradient centrifugation method ( 15 % , 22 % , 30 % , 40 % , 50 % , 60 % ) was introduced to isolate spermatids from the cellular suspensions . the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis , and the viability of spermatogenic cells was assessed by using eosin y exclusion test
在第一部分试验中,首先利用连续3次组合酶消化成年小鼠睾丸制备睾丸细胞悬液,然后经6层非连续percoll梯度离心法( 15 、 22 、 30 、 40 、 50和60 )分离,通过形态学和流式细胞术鉴定南京医科大学硕士学位论文各个percoll组分中精子细胞的含量,并以伊红y排斥试验测定细胞的存活率。